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annexin v complementary dna microarray  (TaKaRa)


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    Structured Review

    TaKaRa annexin v complementary dna microarray
    Annexin V Complementary Dna Microarray, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complementary+dna+(cdna)+microarray/pm20686813-65-19-29
    Average 86 stars, based on 1 article reviews
    annexin v complementary dna microarray - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: Alteration of POLDIP3 Splicing Associated with Loss of Function of TDP-43 in Tissues Affected with ALS
    Article Snippet: .. Full-length human TDP-43 complementary DNA (cDNA) and full-length human POLDIP3 cDNA were isolated from the human whole-brain cDNA library (Clontech, Palo Alto, CA, USA) and subcloned into the pcDNA DEST-40 (Invitrogen, Carlsbad, CA, USA) and Vivid colors pcDNA6.2/EmGFP vector (Invitrogen). .. Human POLDIP3 variant-2 expression plasmid was generated using KOD plus mutagenesis kit (TOYOBO, Osaka, Japan).

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
    Article Snippet: U6 n/a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) Guan et al., 2020* Trizol (Invitrogen, CA, USA) TaqMan MicroRNA Reverse Transcription Kit Universal PCR Master Mix (Applied Biosystems, CA, USA) U6 n/a n/a Guan et al., 2022* TRIzol (Invitrogen, USA) Reverse transcription kit (Takara Bio, China). .. RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR. .. Life Technologies (Thermo scientific, Waltham, MA, USA) Caenorhabditis elegans miR-39 n/a n/a U6 n/a n/a (continued on next page) A .M .

    cDNA Library Assay:

    Article Title: Alteration of POLDIP3 Splicing Associated with Loss of Function of TDP-43 in Tissues Affected with ALS
    Article Snippet: .. Full-length human TDP-43 complementary DNA (cDNA) and full-length human POLDIP3 cDNA were isolated from the human whole-brain cDNA library (Clontech, Palo Alto, CA, USA) and subcloned into the pcDNA DEST-40 (Invitrogen, Carlsbad, CA, USA) and Vivid colors pcDNA6.2/EmGFP vector (Invitrogen). .. Human POLDIP3 variant-2 expression plasmid was generated using KOD plus mutagenesis kit (TOYOBO, Osaka, Japan).

    Plasmid Preparation:

    Article Title: Alteration of POLDIP3 Splicing Associated with Loss of Function of TDP-43 in Tissues Affected with ALS
    Article Snippet: .. Full-length human TDP-43 complementary DNA (cDNA) and full-length human POLDIP3 cDNA were isolated from the human whole-brain cDNA library (Clontech, Palo Alto, CA, USA) and subcloned into the pcDNA DEST-40 (Invitrogen, Carlsbad, CA, USA) and Vivid colors pcDNA6.2/EmGFP vector (Invitrogen). .. Human POLDIP3 variant-2 expression plasmid was generated using KOD plus mutagenesis kit (TOYOBO, Osaka, Japan).

    Reverse Transcription:

    Article Title: Early onset female pattern hair loss: A case-control study for analyzing clinical features and genetic variants.
    Article Snippet: .. Y. Meng et al. BBA - Molecular and Cell Biology of Lipids 1867 (2022) 159120 subunits α1 and α2 were obtained by reverse transcription of oligo (dT)primed human heart total RNA using a PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, Kyoto, Japan). .. Amplification was performed with primers listed in Table 2 and cloned into pGEX-4 T-1 vector (GE Healthcare, Marlborough, MA, USA) using a ClonExpress-II One Step Cloning Kit (Vazyme, Nanjing, China).

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
    Article Snippet: U6 n/a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) Guan et al., 2020* Trizol (Invitrogen, CA, USA) TaqMan MicroRNA Reverse Transcription Kit Universal PCR Master Mix (Applied Biosystems, CA, USA) U6 n/a n/a Guan et al., 2022* TRIzol (Invitrogen, USA) Reverse transcription kit (Takara Bio, China). .. RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR. .. Life Technologies (Thermo scientific, Waltham, MA, USA) Caenorhabditis elegans miR-39 n/a n/a U6 n/a n/a (continued on next page) A .M .

    cDNA Synthesis:

    Article Title: Early onset female pattern hair loss: A case-control study for analyzing clinical features and genetic variants.
    Article Snippet: .. Y. Meng et al. BBA - Molecular and Cell Biology of Lipids 1867 (2022) 159120 subunits α1 and α2 were obtained by reverse transcription of oligo (dT)primed human heart total RNA using a PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, Kyoto, Japan). .. Amplification was performed with primers listed in Table 2 and cloned into pGEX-4 T-1 vector (GE Healthcare, Marlborough, MA, USA) using a ClonExpress-II One Step Cloning Kit (Vazyme, Nanjing, China).

    Article Title: Distinct tumor architectures for metastatic colonization of the brain
    Article Snippet: .. 30 days post-inoculation, mice were intraperitoneally injected with 250 mg/kg 5-fluorocytosine (5-FC) (Sigma Aldrich, Cat: F71291G), and after 12 hours, mouse brains were harvested and homogenized. mRNA was extracted from homogenized cell lysate by 4 × 250 μL/brain Oligo (dT) 25 magnetic beads (New England Biolabs, Cat: S1419S), and immunoprecipitated (IP) with 2.5 μg/brain anti-BrdU antibody (Abcam, Cat: ab6326). cDNA libraries were constructed using the immunoprecipitated mRNA and SMARTer PCR cDNA Synthesis Kit (Clontech, Cat: 634926), and sequenced on a HiSeq 2500 system (Illumina, Integrated Genomics Core, MSKCC). ..

    Article Title: Hepatitis B Virus- KMT2B Integration Drives Hepatic Oncogenic Processes in a Human Gene-edited Induced Pluripotent Stem Cells-derived Model
    Article Snippet: Total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer’s protocols. .. First-strand cDNA was synthesized using a PrimeScript IV 1st strand cDNA Synthesis Mix (Takara Bio) and used as a template for PCR amplification. qPCR analysis was performed using the Universal Probe Library system (Roche Diagnostics) or using a QuantiTect SYBR Green PCR Kit (Qiagen) in a reaction volume of 20 μL. ..

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
    Article Snippet: U6 n/a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) Guan et al., 2020* Trizol (Invitrogen, CA, USA) TaqMan MicroRNA Reverse Transcription Kit Universal PCR Master Mix (Applied Biosystems, CA, USA) U6 n/a n/a Guan et al., 2022* TRIzol (Invitrogen, USA) Reverse transcription kit (Takara Bio, China). .. RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR. .. Life Technologies (Thermo scientific, Waltham, MA, USA) Caenorhabditis elegans miR-39 n/a n/a U6 n/a n/a (continued on next page) A .M .

    Injection:

    Article Title: Distinct tumor architectures for metastatic colonization of the brain
    Article Snippet: .. 30 days post-inoculation, mice were intraperitoneally injected with 250 mg/kg 5-fluorocytosine (5-FC) (Sigma Aldrich, Cat: F71291G), and after 12 hours, mouse brains were harvested and homogenized. mRNA was extracted from homogenized cell lysate by 4 × 250 μL/brain Oligo (dT) 25 magnetic beads (New England Biolabs, Cat: S1419S), and immunoprecipitated (IP) with 2.5 μg/brain anti-BrdU antibody (Abcam, Cat: ab6326). cDNA libraries were constructed using the immunoprecipitated mRNA and SMARTer PCR cDNA Synthesis Kit (Clontech, Cat: 634926), and sequenced on a HiSeq 2500 system (Illumina, Integrated Genomics Core, MSKCC). ..

    Magnetic Beads:

    Article Title: Distinct tumor architectures for metastatic colonization of the brain
    Article Snippet: .. 30 days post-inoculation, mice were intraperitoneally injected with 250 mg/kg 5-fluorocytosine (5-FC) (Sigma Aldrich, Cat: F71291G), and after 12 hours, mouse brains were harvested and homogenized. mRNA was extracted from homogenized cell lysate by 4 × 250 μL/brain Oligo (dT) 25 magnetic beads (New England Biolabs, Cat: S1419S), and immunoprecipitated (IP) with 2.5 μg/brain anti-BrdU antibody (Abcam, Cat: ab6326). cDNA libraries were constructed using the immunoprecipitated mRNA and SMARTer PCR cDNA Synthesis Kit (Clontech, Cat: 634926), and sequenced on a HiSeq 2500 system (Illumina, Integrated Genomics Core, MSKCC). ..

    Immunoprecipitation:

    Article Title: Distinct tumor architectures for metastatic colonization of the brain
    Article Snippet: .. 30 days post-inoculation, mice were intraperitoneally injected with 250 mg/kg 5-fluorocytosine (5-FC) (Sigma Aldrich, Cat: F71291G), and after 12 hours, mouse brains were harvested and homogenized. mRNA was extracted from homogenized cell lysate by 4 × 250 μL/brain Oligo (dT) 25 magnetic beads (New England Biolabs, Cat: S1419S), and immunoprecipitated (IP) with 2.5 μg/brain anti-BrdU antibody (Abcam, Cat: ab6326). cDNA libraries were constructed using the immunoprecipitated mRNA and SMARTer PCR cDNA Synthesis Kit (Clontech, Cat: 634926), and sequenced on a HiSeq 2500 system (Illumina, Integrated Genomics Core, MSKCC). ..

    Construct:

    Article Title: Distinct tumor architectures for metastatic colonization of the brain
    Article Snippet: .. 30 days post-inoculation, mice were intraperitoneally injected with 250 mg/kg 5-fluorocytosine (5-FC) (Sigma Aldrich, Cat: F71291G), and after 12 hours, mouse brains were harvested and homogenized. mRNA was extracted from homogenized cell lysate by 4 × 250 μL/brain Oligo (dT) 25 magnetic beads (New England Biolabs, Cat: S1419S), and immunoprecipitated (IP) with 2.5 μg/brain anti-BrdU antibody (Abcam, Cat: ab6326). cDNA libraries were constructed using the immunoprecipitated mRNA and SMARTer PCR cDNA Synthesis Kit (Clontech, Cat: 634926), and sequenced on a HiSeq 2500 system (Illumina, Integrated Genomics Core, MSKCC). ..

    Polymerase Chain Reaction:

    Article Title: Distinct tumor architectures for metastatic colonization of the brain
    Article Snippet: .. 30 days post-inoculation, mice were intraperitoneally injected with 250 mg/kg 5-fluorocytosine (5-FC) (Sigma Aldrich, Cat: F71291G), and after 12 hours, mouse brains were harvested and homogenized. mRNA was extracted from homogenized cell lysate by 4 × 250 μL/brain Oligo (dT) 25 magnetic beads (New England Biolabs, Cat: S1419S), and immunoprecipitated (IP) with 2.5 μg/brain anti-BrdU antibody (Abcam, Cat: ab6326). cDNA libraries were constructed using the immunoprecipitated mRNA and SMARTer PCR cDNA Synthesis Kit (Clontech, Cat: 634926), and sequenced on a HiSeq 2500 system (Illumina, Integrated Genomics Core, MSKCC). ..

    Article Title: Hepatitis B Virus- KMT2B Integration Drives Hepatic Oncogenic Processes in a Human Gene-edited Induced Pluripotent Stem Cells-derived Model
    Article Snippet: Total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer’s protocols. .. First-strand cDNA was synthesized using a PrimeScript IV 1st strand cDNA Synthesis Mix (Takara Bio) and used as a template for PCR amplification. qPCR analysis was performed using the Universal Probe Library system (Roche Diagnostics) or using a QuantiTect SYBR Green PCR Kit (Qiagen) in a reaction volume of 20 μL. ..

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
    Article Snippet: U6 n/a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) Guan et al., 2020* Trizol (Invitrogen, CA, USA) TaqMan MicroRNA Reverse Transcription Kit Universal PCR Master Mix (Applied Biosystems, CA, USA) U6 n/a n/a Guan et al., 2022* TRIzol (Invitrogen, USA) Reverse transcription kit (Takara Bio, China). .. RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR. .. Life Technologies (Thermo scientific, Waltham, MA, USA) Caenorhabditis elegans miR-39 n/a n/a U6 n/a n/a (continued on next page) A .M .

    Synthesized:

    Article Title: Hepatitis B Virus- KMT2B Integration Drives Hepatic Oncogenic Processes in a Human Gene-edited Induced Pluripotent Stem Cells-derived Model
    Article Snippet: Total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer’s protocols. .. First-strand cDNA was synthesized using a PrimeScript IV 1st strand cDNA Synthesis Mix (Takara Bio) and used as a template for PCR amplification. qPCR analysis was performed using the Universal Probe Library system (Roche Diagnostics) or using a QuantiTect SYBR Green PCR Kit (Qiagen) in a reaction volume of 20 μL. ..

    Amplification:

    Article Title: Hepatitis B Virus- KMT2B Integration Drives Hepatic Oncogenic Processes in a Human Gene-edited Induced Pluripotent Stem Cells-derived Model
    Article Snippet: Total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer’s protocols. .. First-strand cDNA was synthesized using a PrimeScript IV 1st strand cDNA Synthesis Mix (Takara Bio) and used as a template for PCR amplification. qPCR analysis was performed using the Universal Probe Library system (Roche Diagnostics) or using a QuantiTect SYBR Green PCR Kit (Qiagen) in a reaction volume of 20 μL. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Hepatitis B Virus- KMT2B Integration Drives Hepatic Oncogenic Processes in a Human Gene-edited Induced Pluripotent Stem Cells-derived Model
    Article Snippet: Total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer’s protocols. .. First-strand cDNA was synthesized using a PrimeScript IV 1st strand cDNA Synthesis Mix (Takara Bio) and used as a template for PCR amplification. qPCR analysis was performed using the Universal Probe Library system (Roche Diagnostics) or using a QuantiTect SYBR Green PCR Kit (Qiagen) in a reaction volume of 20 μL. ..

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
    Article Snippet: U6 n/a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) Guan et al., 2020* Trizol (Invitrogen, CA, USA) TaqMan MicroRNA Reverse Transcription Kit Universal PCR Master Mix (Applied Biosystems, CA, USA) U6 n/a n/a Guan et al., 2022* TRIzol (Invitrogen, USA) Reverse transcription kit (Takara Bio, China). .. RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR. .. Life Technologies (Thermo scientific, Waltham, MA, USA) Caenorhabditis elegans miR-39 n/a n/a U6 n/a n/a (continued on next page) A .M .

    SYBR Green Assay:

    Article Title: Hepatitis B Virus- KMT2B Integration Drives Hepatic Oncogenic Processes in a Human Gene-edited Induced Pluripotent Stem Cells-derived Model
    Article Snippet: Total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer’s protocols. .. First-strand cDNA was synthesized using a PrimeScript IV 1st strand cDNA Synthesis Mix (Takara Bio) and used as a template for PCR amplification. qPCR analysis was performed using the Universal Probe Library system (Roche Diagnostics) or using a QuantiTect SYBR Green PCR Kit (Qiagen) in a reaction volume of 20 μL. ..

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
    Article Snippet: U6 n/a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) Guan et al., 2020* Trizol (Invitrogen, CA, USA) TaqMan MicroRNA Reverse Transcription Kit Universal PCR Master Mix (Applied Biosystems, CA, USA) U6 n/a n/a Guan et al., 2022* TRIzol (Invitrogen, USA) Reverse transcription kit (Takara Bio, China). .. RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR. .. Life Technologies (Thermo scientific, Waltham, MA, USA) Caenorhabditis elegans miR-39 n/a n/a U6 n/a n/a (continued on next page) A .M .

    other:

    Article Title: Distinct but interchangeable subpopulations of colorectal cancer cells with different growth fates and drug sensitivity
    Article Snippet: For generating the pLX304-EGFP vector, the EGFP sequence was transferred from the pDONR221_EGFP plasmid (Addgene #25899, a gift from David Root) into the pLX304 plasmid by the Gateway cloning system (Thermo Fisher Scientific, 11791020).

    Quantitative RT-PCR:

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
    Article Snippet: U6 n/a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) Guan et al., 2020* Trizol (Invitrogen, CA, USA) TaqMan MicroRNA Reverse Transcription Kit Universal PCR Master Mix (Applied Biosystems, CA, USA) U6 n/a n/a Guan et al., 2022* TRIzol (Invitrogen, USA) Reverse transcription kit (Takara Bio, China). .. RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR. .. Life Technologies (Thermo scientific, Waltham, MA, USA) Caenorhabditis elegans miR-39 n/a n/a U6 n/a n/a (continued on next page) A .M .

    Microarray:

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
    Article Snippet: U6 n/a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) Guan et al., 2020* Trizol (Invitrogen, CA, USA) TaqMan MicroRNA Reverse Transcription Kit Universal PCR Master Mix (Applied Biosystems, CA, USA) U6 n/a n/a Guan et al., 2022* TRIzol (Invitrogen, USA) Reverse transcription kit (Takara Bio, China). .. RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR. .. Life Technologies (Thermo scientific, Waltham, MA, USA) Caenorhabditis elegans miR-39 n/a n/a U6 n/a n/a (continued on next page) A .M .

    Article Title: Applications of microarray technology in breast cancer research
    Article Snippet: .. Differential expression between hormone-refractory CWR22R prostate cancer xenografts and xenografts of the parental hormone-sensitive CWR22 strain was examined using a 588 cDNA Clontech filter microarray. ..

    Purification:

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
    Article Snippet: U6 n/a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) Guan et al., 2020* Trizol (Invitrogen, CA, USA) TaqMan MicroRNA Reverse Transcription Kit Universal PCR Master Mix (Applied Biosystems, CA, USA) U6 n/a n/a Guan et al., 2022* TRIzol (Invitrogen, USA) Reverse transcription kit (Takara Bio, China). .. RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR. .. Life Technologies (Thermo scientific, Waltham, MA, USA) Caenorhabditis elegans miR-39 n/a n/a U6 n/a n/a (continued on next page) A .M .

    Labeling:

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
    Article Snippet: U6 n/a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) Guan et al., 2020* Trizol (Invitrogen, CA, USA) TaqMan MicroRNA Reverse Transcription Kit Universal PCR Master Mix (Applied Biosystems, CA, USA) U6 n/a n/a Guan et al., 2022* TRIzol (Invitrogen, USA) Reverse transcription kit (Takara Bio, China). .. RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR. .. Life Technologies (Thermo scientific, Waltham, MA, USA) Caenorhabditis elegans miR-39 n/a n/a U6 n/a n/a (continued on next page) A .M .

    Multiplex Assay:

    Article Title: Relationship between microRNA expression profiling and gestational diabetes: A systematic review
    Article Snippet: U6 n/a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) Guan et al., 2020* Trizol (Invitrogen, CA, USA) TaqMan MicroRNA Reverse Transcription Kit Universal PCR Master Mix (Applied Biosystems, CA, USA) U6 n/a n/a Guan et al., 2022* TRIzol (Invitrogen, USA) Reverse transcription kit (Takara Bio, China). .. RT-qPCR U6 n/a n/a HUANG et al., 2021* Trizol n/a RT-qPCR GAPDH n/a n/a Li 2015ɨ** RecoverAllTM Total Nucleic Acid Isolation kit (Ambion) y TRNzol reagent (TIANGEN Biotech, Beijing, China) miRNA first-strand cDNA kit (TIANGEN Biotech, Beijing, China) miRcute miRNA qPCR detection kit (TIANGEN Biotech, Beijing, China) U6 Agilent Human miRNA Microarray (Agilent) n/a Li et al., 2018** KingFisher Automated Purification Kits (Thermo Fisher) miRCURY LNATM microRNA Array Hi-Power Labeling kit (Qiagen) n/a Hairpin-itTMmiRNAs RealTime PCR Quantization Kit (GenePharma) n/a n/a n/a Li W 2022* TRIzol reagent (Magen Biotechnology Co.) TaKaRa PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) miScript SYBR Green PCR Kit (TaKaRa) U6 n/a n/a Peng et al., 2019* Trizol (Ambion, USA) miRcute miRNA FirstStrand cDNA Synthesis Kit (TianGen, Beijing, China) miRcute miRNA qPCR Detection Kit (SYBR Green) (TianGen, Beijing China) U6 n/a n/a Sun et al., 2020* Trizol reagent (Invitrogen, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan Univel PCR Master Mix (Applied Biosystems, Foster City, CA, USA) U6 n/a n/a Tang 2020ᵻ* Trizol Reagent (Invitrogen, Carlsbad, CA, USA) M-MLV Reverse Transcriptase (Invitrogen) qRT–PCR (BIONEER, Daejeon, Korea) U6 n/a NEBNext Multiplex Small RNA Library Prep Set for Illumina_x0003_ (NEB, Ipswich, MA, USA) Wang S 2020* Trizol reagent (Invitrogen) Transcriptase Kit (Takara, Otsu, Japan) SYBR Green PCR Master Mix (Takara) U6 n/a n/a Zhang Lu 2021* Trizol n/a RT-qPCR U6 n/a n/a Zhao et al., 2014* Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) TaqMan MicroRNA Reverse Transcription kit TaqMan microRNA assay (Applied Biosystems, Branchburg, NJ, USA) U6 n/a n/a Serum Abdeltawab et al., 2020** miRVana PARIS kits (Life Technologies, Grand Island, NY, USA) TaqMan MicroRNA Reverse Transcription Kit TaqMan quantitative RTPCR. .. Life Technologies (Thermo scientific, Waltham, MA, USA) Caenorhabditis elegans miR-39 n/a n/a U6 n/a n/a (continued on next page) A .M .

    Quantitative Proteomics:

    Article Title: Applications of microarray technology in breast cancer research
    Article Snippet: .. Differential expression between hormone-refractory CWR22R prostate cancer xenografts and xenografts of the parental hormone-sensitive CWR22 strain was examined using a 588 cDNA Clontech filter microarray. ..



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